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KMID : 0545120060160091477
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Journal of Microbiology and Biotechnology
2006 Volume.16 No. 9 p.1477 ~ p.1480
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Functional Dissection of Sigma-Like Domain in Antibiotic Regulatory Gene, afsR2 in Streptomyces lividans
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Kim Chang-Young
Park Hyun-Joo
Kim Eung-Soo
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Abstract
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The 63-amino-acid-encoding afsR2 is a global antibiotics-stimulating regulatory gene identified from the chromosome of Streptomyces lividans. To dissect a putative functional domain in afsR2, several afsR2-derivative deletion constructs were generated and screened for the loss of actinorhodin-stimulating capability. The afsR2-derivative construct missing a 50-bp C-terminal region significantly lost its actinorhodin-stimulating capability in S. lividans. In addition, site-directed mutagenesis on amino acid positions of #57-#61 in a 50-bp C-terminal region, some of which are conserved among known Sigma 70 family proteins, significantly changed the AfsR2's activity. These results imply that the C-terminal region of AfsR2 is functionally important for antibiotics-stimulating capability and the regulatory mechanism might be somehow related to the sigma-like domain present in the C-terminal of AfsR2.
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KEYWORD
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sigma factor, Streptomyces, afsR2, antibiotic regulation
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